{"id":206,"date":"2023-05-25T12:04:52","date_gmt":"2023-05-25T12:04:52","guid":{"rendered":"https:\/\/research.ubbcluj.ro\/infrastructure\/?p=206"},"modified":"2023-06-14T10:28:32","modified_gmt":"2023-06-14T10:28:32","slug":"fluorescence-microscopy-platform","status":"publish","type":"post","link":"https:\/\/research.ubbcluj.ro\/infrastructure\/fluorescence-microscopy-platform\/","title":{"rendered":"Fluorescence Microscopy platform"},"content":{"rendered":"\n<p>The Time-resolved confocal fluorescence microscope is adapted for different types of measurements\/modes of data analysis: FLIM, FCS, FRET, measurement of fluorescence lifetimes in liquid or solid samples, unimolecular spectroscopy, nonlinear optics measurements, monitoring of fluorescence signal fluctuations. Possible applications: FLIM on biological structures (cells, tissue) or materials with luminescent properties; characterization of the interaction of some exo- or endogenous chromophores with (plasmonic) nanoparticles applied in therapy, diagnosis and imaging; Toxicity assessments; Molecular fluorescence correlation studies; Sensoristics, Biodetection, Quantitative microscopy studies; Cell biology; Non-linear optics; Two-photon imaging; Materials science; The confocal microscopy system (Re-scan Confocal Microscopy &#8211; RCM) is based on the &amp;quot;double scan&amp;quot; method (a scan of the laser beam simultaneously with a scan of the beam emitted by the sample). This innovative method leads to obtaining a much better lateral resolution than in standard confocal microscopy. This peculiarity of scanning and respectively high resolution gives to our microscopy system a high degree of uniqueness both nationally and internationally; 2 re-scanning RCM units: for the NIR domain, with lateral resolution of 240 nm (RCM-NIR) and 140 nm respectively for the visible domain (RCM-VIS); the RCM-NIR unit is equipped with two laser diodes with emission lines at 640 and 785 nm, respectively.<\/p>\n","protected":false},"excerpt":{"rendered":"<p>The Time-resolved confocal fluorescence microscope is adapted for different types of measurements\/modes of data analysis: FLIM, FCS, FRET, measurement of fluorescence lifetimes in liquid or &hellip;<\/p>\n","protected":false},"author":1,"featured_media":256,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[13],"tags":[],"class_list":["post-206","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-fluorescence-microscopy-platform"],"_links":{"self":[{"href":"https:\/\/research.ubbcluj.ro\/infrastructure\/wp-json\/wp\/v2\/posts\/206","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/research.ubbcluj.ro\/infrastructure\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/research.ubbcluj.ro\/infrastructure\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/research.ubbcluj.ro\/infrastructure\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/research.ubbcluj.ro\/infrastructure\/wp-json\/wp\/v2\/comments?post=206"}],"version-history":[{"count":1,"href":"https:\/\/research.ubbcluj.ro\/infrastructure\/wp-json\/wp\/v2\/posts\/206\/revisions"}],"predecessor-version":[{"id":207,"href":"https:\/\/research.ubbcluj.ro\/infrastructure\/wp-json\/wp\/v2\/posts\/206\/revisions\/207"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/research.ubbcluj.ro\/infrastructure\/wp-json\/wp\/v2\/media\/256"}],"wp:attachment":[{"href":"https:\/\/research.ubbcluj.ro\/infrastructure\/wp-json\/wp\/v2\/media?parent=206"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/research.ubbcluj.ro\/infrastructure\/wp-json\/wp\/v2\/categories?post=206"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/research.ubbcluj.ro\/infrastructure\/wp-json\/wp\/v2\/tags?post=206"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}