{"id":257,"date":"2023-06-14T10:32:20","date_gmt":"2023-06-14T10:32:20","guid":{"rendered":"https:\/\/research.ubbcluj.ro\/infrastructure\/?p=257"},"modified":"2023-06-14T10:36:42","modified_gmt":"2023-06-14T10:36:42","slug":"re-scanning-confocal-fluorescence-microscope-system-rcm","status":"publish","type":"post","link":"https:\/\/research.ubbcluj.ro\/infrastructure\/re-scanning-confocal-fluorescence-microscope-system-rcm\/","title":{"rendered":"Re-Scanning Confocal Fluorescence Microscope system (RCM)"},"content":{"rendered":"\n

Research in Nanobiophotonics and Laser Microspectroscopy Center<\/strong><\/p>\n\n\n\n

(https:\/\/www.nanobiophotonics.ro\/) is focused on the synthesis, fabrication, characterization and biofunctionalization of optically active noble-metal nanoparticles and versatile hybrid nanostructures and nanocomposites (biopolymers \/ proteins \/ fluorophores \/ graphene \/ semiconductor) driven by new properties and functions toward biomedical and technological applications and societal interest. Specifically, we develop nanotherapeutic systems based on drug-incorporated biopolymer-coated plasmonic nanoparticles, plasmon-enhanced photodynamic therapy and plasmon-induced photothermal therapy combined with multimodal cellular imaging via scanning confocal Raman\/fluorescence microscopy, including SERS reporters and fluorescent contrast agents, time-resolved fluorescence (FLIM). Also, we implement plasmonic-based biosensing and diagnostic applications via SERS, local surface plasmon resonance (LSPR) and metal-enhanced fluorescence (MEF). As we believe in collaboration and interdisciplinarity, together with our partners (physicists, chemists, biochemists, biologists, oncologists, surgeons, histopathologists, etc. ) from recognized research centers in Romania and abroad, we aim to develop new solutions to research beyond the traditional disciplinary boundaries of physics, chemistry and biology.<\/p>\n\n\n\n

The RCM system<\/strong> enables to investigate live-cell dynamics in high resolution while keeping the laser power extremely low. In particular, our RCM-NIR unit allows deep tissue biomedical imaging, achieving much higher sensitivity in the near-infrared wavelengths. It works with 640 nm and 785 nm wavelengths which, due to the longer wavelength, are less phototoxic and penetrate much better into biological samples.<\/p>\n\n\n\n

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Applicability: <\/strong>High-resolution confocal fluorescence (bio)imaging RCM, respectively epi-fluorescence microscopy on biological systems (cells, tissue, “phantoms”) or luminescent materials in Vis-NIR of interest in medicine, biology, photonics, materials science, etc.; investigation of fluorescent molecules, nanoclusters and nanoparticles; investigating the distribution of fluorescent markers in living cells of interest at a high spatial resolution; collection of 3D images from cells, tissues, phantoms, etc.; automatic multi-mode image acquisitions.<\/p>\n\n\n\n

Performances: <\/strong>This confocal microscopy system (Re-scan Confocal Microscopy – RCM) is based on the “double scan” method (a scan of the laser beam simultaneously with a scan of the beam emitted by the sample). This innovative method leads to obtaining a much better lateral resolution than in standard confocal microscopy (the working principle of the RCM is presented at https:\/\/www.confocal.nl\/. This peculiarity of scanning and respectively high resolution gives to our microscopy system a high degree of uniqueness both nationally and internationally; Characteristics of RCM-VIS unit (visible range): Maximum scanning speed: 1 fps at 512×512 maximum resolution (RCM resolution) \/ 4 fps at 512×512 through the binning process ( low resolution); Resolution: 170 nm (no deconvolution); Pinhole size: fixed, 50 \u00b5m; Field of view: 88 x 88 \u00b5m at 100x magnification; Characteristics of RCM-NIR unit (near infrared (NIR) range):<\/p>\n\n\n\n

Maximum scan speed: 1 fps at 512×512 maximum resolution (RCM resolution) \/ 4 fps at 512×512 binning (reduced resolution), Field of view: 88 x 88 \u00b5m at 100x magnification<\/p>\n\n\n\n

KEYWORDS<\/strong>: High-resolution confocal fluorescence (bio)imaging, 3D fluorescence imaging, NIR confocal imaging,<\/p>\n\n\n\n

Key Contact:<\/strong> Monica Focsan (monica.iosin@ubbcluj.ro<\/a>)<\/p>\n\n\n\n

Website:<\/strong> https:\/\/www.nanobiophotonics.ro\/files\/product_doc\/2310\/product-63e2119d84e5d.pdf<\/a><\/p>\n\n\n\n

Access Policy<\/strong><\/p>\n\n\n\n

Available for a pre-evaluation of the complexity of the samples and estimation of the working time, in the 9:30 – 17:30 interval, based on a preliminary email.<\/p>\n\n\n\n

Usage conditions: exclusively by the personnel responsible for the mentioned specialty.<\/p>\n\n\n\n

Analysis price – extern UBB: Preliminary evaluation \u2013 free of charge; Recording and analysis of confocal fluorescence image: 350 lei\/sample; Recording and analysis of 3D confocal fluorescence: 500 Lei \/sample Analysis price – intern UBB: free of charge.<\/p>\n\n\n\n

Lista articole RCM<\/strong><\/p>\n\n\n\n

1. NIR photothermal-activable drug-conjugated microcapsules for in vitro targeted delivery and release: an alternative treatment of diabetic retinopathy, D. Stoia, M. Nistor, M. Suciu, R. Borlan, A. Campu, D. Rugina, D. Maniu, S. Astilean, M. Focsan, Int. J. Pharm, 635 (2023) 122700<\/p>\n\n\n\n

2. Glutathione-capped gold nanoclusters as near-infrared-emitting efficient contrast agents for confocal fluorescence imaging of tissue-mimicking phantoms, A.-M. Hada, A.-M. Craciun, M. Focsan, A. Vulpoi, E.-L. Borcan, S. Astilean, Microchimica Acta, 189 (2022) 337<\/p>\n\n\n\n

3. Hybrid Polymeric Therapeutic Microcarriers for Thermoplasmonic-Triggered Release of Resveratrol, D. Stoia, R. Pop, A. Campu, M. Nistor, S. Astilean, A. Pintea, M. Suciu, D. Rugina, M. Focsan, Colloid Surf. B, 220 (2022) 112915<\/p>\n\n\n\n

4. Probing polyvinylpyrrolidone-passivated graphene oxide nanoflakes as contrast agents inside tissue-like phantoms via multimodal confocal microscopy, M. Potara, S. Suarasan, A-M. Craciun, M. Focsan, A-M. Hada, S. Astilean, Talanta, 247 (2022) 123581<\/p>\n\n\n\n

5. Folate-targeted Pluronic-chitosan nanocapsules loaded with IR780 for near-infrared fluorescence imaging and photothermal-photodynamic therapy of ovarian cancer, M. Potara, T. Nagy-Simon, M. Focsan, E. Licarete, O. Soritau, A. Vulpoi, S. Astilean, Colloids Surf. B Biointerfaces, 203 (2021) 111755<\/p>\n\n\n\n

6. Pluronic stabilized conjugated polymer nanoparticles for NIR fluorescence imaging and dual phototherapy applications, T. Nagy-Simon, O. Diaconu, M. Focsan, A. Vulpoi, I. Botiz, A.-M. Craciun, J. Mol. Struc., 1243 (2021) 130931<\/p>\n\n\n\n

7. Design of Fluorophore Loaded Human Serum Albumin Nanoparticles for Specific Targeting of NIH:OVCAR3 Ovarian Cancer Cells, R. Borlan, A. S. Tatar, O. Soritau, D. Maniu, G. Marc, A. Florea, M. Focsan, S. Astilean, Nanotechnology, 31 (2020) 315102<\/p>\n","protected":false},"excerpt":{"rendered":"

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