{"id":261,"date":"2023-06-14T10:35:45","date_gmt":"2023-06-14T10:35:45","guid":{"rendered":"https:\/\/research.ubbcluj.ro\/infrastructure\/?p=261"},"modified":"2023-06-14T10:36:01","modified_gmt":"2023-06-14T10:36:01","slug":"time-resolved-confocal-fluorescence-microscope-system-flim-microtime-200-picoquant","status":"publish","type":"post","link":"https:\/\/research.ubbcluj.ro\/infrastructure\/time-resolved-confocal-fluorescence-microscope-system-flim-microtime-200-picoquant\/","title":{"rendered":"Time-resolved confocal fluorescence microscope system (FLIM) (MicroTime 200, PicoQuant)"},"content":{"rendered":"\n

Research in Nanobiophotonics and Laser Microspectroscopy Center<\/strong><\/p>\n\n\n\n

(https:\/\/www.nanobiophotonics.ro\/) is focused on the synthesis, fabrication, characterization and biofunctionalization of optically active noble-metal nanoparticles and versatile hybrid nanostructures and nanocomposites (biopolymers \/ proteins \/ fluorophores \/ graphene \/ semiconductor) driven by new properties and functions toward biomedical and technological applications and societal interest. Specifically, we develop nanotherapeutic systems based on drug-incorporated biopolymer-coated plasmonic nanoparticles, plasmon-enhanced photodynamic therapy and plasmon-induced photothermal therapy combined with multimodal cellular imaging via scanning confocal Raman\/fluorescence microscopy, including SERS reporters and fluorescent contrast agents, time-resolved fluorescence (FLIM). Also, we implement plasmonic-based biosensing and diagnostic applications via SERS, local surface plasmon resonance (LSPR) and metal-enhanced fluorescence (MEF). As we believe in collaboration and interdisciplinarity, together with our partners (physicists, chemists, biochemists, biologists, oncologists, surgeons, histopathologists, etc. ) from recognized research centers in Romania and abroad, we aim to develop new solutions to research beyond the traditional disciplinary boundaries of physics, chemistry and biology.<\/p>\n\n\n\n

The FLIM system<\/strong> is adapted for different types of measurements\/modes of data analysis: FLIM, FCS, FRET, measurement of fluorescence lifetimes in liquid or solid samples, unimolecular spectroscopy, nonlinear optics measurements, monitoring of fluorescence signal fluctuations. Possible applications: FLIM on biological structures (cells, tissue) or materials with luminescent properties; characterization of the interaction of some exo- or endogenous chromophores with (plasmonic) nanoparticles applied in therapy, diagnosis and imaging; Toxicity assessments; Molecular fluorescence correlation studies; Sensoristics, Biodetection, Quantitative microscopy studies; Cell biology; Non-linear optics; Two-photon imaging; Materials science<\/p>\n\n\n\n

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Performances:<\/strong> temporal resolution in the picosecond range; high scanning resolution; flexible design;  accessible optical drive; wide range of excitation wavelengths; unimolecular detection sensitivity; two  simultaneous detection channels; friendly data acquisition and processing software; the system is adapted  for different types of measurements\/modes of data analysis: FLIM, FCS, FRET, measurement of fluorescence lifetimes in liquid or solid samples, unimolecular spectroscopy, nonlinear optics measurements, monitoring of fluorescence signal fluctuations, etc.<\/p>\n\n\n\n

KEYWORDS<\/strong>: High-resolution confocal fluorescence (bio)imaging, 3D fluorescence imaging, NIR confocal imaging, FLIM, FRET, FCS, two-photon excitation, nonlinear optics, unimolecular detection sensitivity<\/p>\n\n\n\n

Key Contact:<\/strong> Monica Focsan (monica.iosin@ubbcluj.ro<\/a>)<\/p>\n\n\n\n

Website:<\/strong> https:\/\/www.nanobiophotonics.ro\/files\/product_doc\/2\/product-63e216fc48756.pdf<\/a><\/p>\n\n\n\n

Access Policy:<\/strong><\/p>\n\n\n\n

Availability for Access and Use Contact persons: Monica Foc\u015fan, CS I (email: monica.iosin@ubbcluj.ro, 0264454554\/int 116) Ana-Maria Cr\u0103ciun, CS III (email: ana.gabudean@ubbcluj.ro, 0264454554\/int 116) Available for a pre-evaluation of the complexity of the samples and estimation of the working time, in the 9:30 – 17:30 interval, based on a preliminary email or phone appointment. Usage conditions: exclusively by the personnel responsible for the mentioned specialty<\/p>\n\n\n\n

Analysis price – extern UBB: Preliminary evaluation \u2013 free of charge; Recording and analysis of FLIM image: 300 lei\/sample; Spectrum measurement: 250 lei\/sample.<\/p>\n\n\n\n

Analysis price – intern UBB: free of charge<\/p>\n\n\n\n

Lista articole FLIM<\/strong><\/p>\n\n\n\n

1. New fluorescent electrospun polymer materials containing phenothiazinyl carboxylate metal salts for versatile latent fingerprint detection, M. Gal, C. Cristea, A.M. Craciun, A. Turza, L. Barbu-Tudoran, B. Balazs, T. Lovasz, L. Silaghi-Dumitrescu, L. Gaina, Dyes and Pigments, 2023, 111085.<\/p>\n\n\n\n

2. Glutathione-capped gold nanoclusters as near-infrared-emitting efficient contrast agents for confocal fluorescence imaging of tissue-mimicking phantoms, A.-M. Hada, A.-M. Craciun, M. Focsan, A. Vulpoi, E.-L. Borcan, S. Astilean, Microchimica Acta, 189 (2022) 337<\/p>\n\n\n\n

3. Portable Plasmonic Paper-Based Biosensor for Simple and Rapid Indirect Detection of CEACAM5 Biomarker via Metal-Enhanced Fluorescence, L. Susu, A. Vulpoi, S. Astilean, M. Focsan, Int. J. Mol. Sci., 23 (2022) 11982<\/p>\n\n\n\n

4. Probing polyvinylpyrrolidone-passivated graphene oxide nanoflakes as contrast agents inside tissue-like phantoms via multimodal confocal microscopy, M. Potara, S. Suarasan, A-M. Craciun, M. Focsan, A-M. Hada, S. Astilean, Talanta, 247 (2022) 123581<\/p>\n\n\n\n

5. One-photon excited photoluminescence of gold nanospheres and its application in prostate specific antigen detection via fluorescence correlation spectroscopy (FCS), A-M Craciun, S. Suarasan, M. Focsan, S. Astilean, Talanta, 228 (2021) 122242<\/p>\n\n\n\n

6. Two-photon excited photoluminescence lifetime imaging studies on individual gelatin-coated gold nanorods, A. M. Craciun, L. Susu, M. Baia, J. Molec. Struct., 1243 (2021) 130785<\/p>\n\n\n\n

7. Emese Gal, Balazs Brem, Luiza Ioana Gaina, Ana-Maria Craciun, Castelia Cristea, Luminita Silaghi-Dumitrescu, Optical properties of new 5-(phenothiazinyl) methylidenebarbituric acid derivatives, J. Mol. Struct. 1247 (2021) 131334<\/p>\n\n\n\n

8. Melinda Gal, Castelia Cristea, Tamas Lovasz, Ana-Maria Craciun,, Alexandru Turza, Dan Porumb, Emese Gal, Gabriel Katona, Luminita Silaghi-Dumitrescu, Luiza Gaina, New fluorescent phenothiazine carboxylates for fluorescent nanomaterials, J. Mol. Struct. 1246 (2021) 131174<\/p>\n\n\n\n

9. Gold nanoclusters performing as contrast agents for non-invasive imaging of tissue-like phantoms via two-photon excited fluorescence lifetime imaging, A.-M. Hada, A. M. Craciun, S. Astilean, Analyst, 146 (2021) 7126-7130<\/p>\n\n\n\n

10. Novel (Phenothiazinyl)Vinyl-Pyridinium Dyes and Their Potential Applications as Cellular Staining Agents, B. Stoean, D. Rugina, M. Focsan, A-M. Craciun, M. Nistor, T. Lovasz, A. Turza, I-D. Porumb, E. G\u00e1l, C. Cristea, L. Silaghi-Dumitrescu, S. Astilean and L. Gaina, Int. J. Mol. Sci., 22 (2021) 2985<\/p>\n\n\n\n

11. Intrinsic Photoluminescence of Solid-State Gold Nanoclusters: Towards Fluorescence Lifetime Imaging of Tissue-Like Phantoms Under Two-Photon Near-Infrared Excitation, A.-M. Hada, A. M. Craciun, S. Astilean, Front. Chem., 9 (2021) 761711<\/p>\n\n\n\n

12. Folic acid functionalized gold nanoclusters for enabling targeted fluorescence imaging of human ovarian cancer cells, A.-M. Hada, A.-M. Craciun, M. Focsan, R. Borlan, O. Soritau, M. Todea, S. Astilean, Talanta, 225 (2021) 121960<\/p>\n\n\n\n

13. Antibody Functionalized Theranostic Protein Nanoparticles for Synergistic Deep Red Fluorescence Imaging and Multimodal Therapy of Ovarian Cancer, R. Borlan, M. Focsan, M. Perde-Schrepler, O. Soritau, A. Campu, L. Gaina, E. Pall, B. Pop, O. Baldasici, C. Gherman, D. Stoia, D. Maniu, S. Astilean, Biomaterials Science, 9 (2021) 6183-6202<\/p>\n\n\n\n

14. Fluorescent Polyelectrolyte System to Track Anthocyanins Delivery inside Melanoma Cells, R. Ghiman, M. Nistor, M. Foc\u0219an*, A. Pintea, S. A\u0219tilean and D. Rugina, Nanomaterials, 11 (2021) 782<\/p>\n\n\n\n

15. Pluronic stabilized conjugated polymer nanoparticles for NIR fluorescence imaging and dual phototherapy applications, T. Nagy-Simon, O. Diaconu, M. Focsan, A. Vulpoi, I. Botiz, A.-M. Craciun, J. Mol. Struc., 1243 (2021) 130931<\/p>\n\n\n\n

16. Surface-enhanced fluorescence imaging on linear arrays of plasmonic half-shells, Cosmin Farcau, Ana-Maria Craciun, Renaud A. L. Vall\u00e9e, J. Chem. Phys., 153 (2020) 164701<\/p>\n\n\n\n

17. Intracellular Fate and Impact on Gene Expression of Doxorubicin\/Cyclodextrin-Graphene Nanomaterials at Sub-Toxic Concentration, D. Caccamo, M. Curr\u00f2, R. Ientile, E. Verderio, A. Scala, A. Mazzaglia, R. Pennisi, M. Musarra-Pizzo, R. Zagami, G. Neri, C. Rosmini, M. Potara, M. Focsan, S. Astilean, A. Piperno, M. Sciortino, Int. J. Mol. Sci., 21 (2020) 4891<\/p>\n","protected":false},"excerpt":{"rendered":"

Research in Nanobiophotonics and Laser Microspectroscopy Center (https:\/\/www.nanobiophotonics.ro\/) is focused on the synthesis, fabrication, characterization and biofunctionalization of optically active noble-metal nanoparticles and versatile hybrid …<\/p>\n","protected":false},"author":1,"featured_media":256,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[13],"tags":[],"class_list":["post-261","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-fluorescence-microscopy-platform"],"_links":{"self":[{"href":"https:\/\/research.ubbcluj.ro\/infrastructure\/wp-json\/wp\/v2\/posts\/261","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/research.ubbcluj.ro\/infrastructure\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/research.ubbcluj.ro\/infrastructure\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/research.ubbcluj.ro\/infrastructure\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/research.ubbcluj.ro\/infrastructure\/wp-json\/wp\/v2\/comments?post=261"}],"version-history":[{"count":2,"href":"https:\/\/research.ubbcluj.ro\/infrastructure\/wp-json\/wp\/v2\/posts\/261\/revisions"}],"predecessor-version":[{"id":264,"href":"https:\/\/research.ubbcluj.ro\/infrastructure\/wp-json\/wp\/v2\/posts\/261\/revisions\/264"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/research.ubbcluj.ro\/infrastructure\/wp-json\/wp\/v2\/media\/256"}],"wp:attachment":[{"href":"https:\/\/research.ubbcluj.ro\/infrastructure\/wp-json\/wp\/v2\/media?parent=261"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/research.ubbcluj.ro\/infrastructure\/wp-json\/wp\/v2\/categories?post=261"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/research.ubbcluj.ro\/infrastructure\/wp-json\/wp\/v2\/tags?post=261"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}