{"id":51,"date":"2023-03-14T18:30:41","date_gmt":"2023-03-14T18:30:41","guid":{"rendered":"https:\/\/research.ubbcluj.ro\/infrastructure\/?p=51"},"modified":"2023-04-11T07:56:36","modified_gmt":"2023-04-11T07:56:36","slug":"echipmanet-2","status":"publish","type":"post","link":"https:\/\/research.ubbcluj.ro\/infrastructure\/echipmanet-2\/","title":{"rendered":"EPR spectroscopy"},"content":{"rendered":"\n

Research centers<\/strong><\/h2>\n\n\n\n

The Laboratory of EPR spectroscopy is overseen jointly by researchers from the The Center for Fundamental and Applied Heterochemistry \u2013 Metallomica<\/a>, the Computer Aided Process Engineering Research Centre<\/a> and the Institute of Physics \u201cIoan Ursu\u201d<\/a>.<\/p>\n\n\n\n

The Laboratory has a list of core personnel from these research units as detailed below, in addition to postdoctoral researchers and students that operate the instruments on a temporaray basis.<\/p>\n\n\n\n

Permanent team<\/strong><\/p>\n\n\n\n

Prof.  Radu Silaghi-Dumitrescu, chemist, with direct experience in applying EPR\/ESR techniques in the biomedical and materials science area.<\/p>\n\n\n\n

Prof. Grigore Damian (grigore.damian@ubbcluj.ro<\/a>), physicist, experienced in EPR\/ESR spectroscopy techniques and methods in material physics and biophysics.<\/p>\n\n\n\n

Prof. Vasile Micl\u0103u\u0219 (vasile.miclaus@ubbcluj.ro<\/a>), chemical engineer with experience in EPR\/ESR spectroscopy in inorganic and organic compounds.<\/p>\n\n\n\n

Prof. Augustin Mo\u021b (augustin.mot@ubbcluj.ro<\/a>) is specialized in analytical chemistry, biochemistry and bioinorganic chemistry.<\/p>\n\n\n\n

Prof. Raluca Mereu (Raluca.mereu@ubbcluj.ro<\/a>) is a chemical engineer specialized in materials science.<\/p>\n\n\n\n

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Research infrastructure<\/strong><\/h2>\n\n\n\n

The EPR laboratory`s main objective is research in the field of electron spin resonance\/electron paramagnetic resonance. The target is advanced research with applications in materials science, physics, chemistry, biology, medicine, environment \u2013 using electron spin resonance\/electron paramagnetic resonance (EPR, ESR), including pulsed procedures such as Electron Spin Echo Envelope Modulaton (ESEEM), 2D Hyperfine Sub-level CORrElation (HYSCORE), ENDOR, PELDOR as well as experiments at variable temperature (liquid nitrogen and helium).<\/p>\n\n\n\n

A freeze-quench setup coupled with stopped-flow-UV-vis is also available for preparing samples that require fast (down to 30 ms) freezing.<\/p>\n\n\n\n

Access policy and costs<\/strong><\/h2>\n\n\n\n

Access to the facility (ELEXSYS 580 and\/or EMXmicro) is free of charge. The measurements are performed by the core personnel, unless otherwise agreed with collaborators\/users. For more extensive collaborations, as well as for contracts\/collaborations with third parties, protocols may be negotiated regarding the operator of the instrument, types of experiments and their length, costs, and other aspects.<\/p>\n\n\n\n

Contact person<\/strong><\/h2>\n\n\n\n

Name: Prof. Dr. Radu Silaghi-Dumitrescu<\/p>\n\n\n\n

Affiliation: Babes-Bolyai University, Faculty of Chemistry and Chemical Engineering, Department of Chemistry<\/p>\n\n\n\n

Email: radu.silaghi@ubbcluj.ro<\/a><\/p>\n\n\n\n

Website: http:\/\/www.chem.ubbcluj.ro\/~rsd\/<\/a><\/p>\n\n\n\n

Research and publications<\/strong><\/h2>\n\n\n\n

The main activities of the Center are research-related. Contract-based services are also expected. Any interested parts are welcome to approach the laboratory director in order to discuss either line of collaboration.<\/p>\n\n\n\n

    \n
  • Lehene, Maria; Plesa, Diana; Ionescu-Zinca, Stefania; Iancu, Stefania D.; Leopold, Nicolae; Makarov, Sergei V.; Br\u00e2nzanic,  Adrian M.V.; Silaghi-Dumitrescu, Radu. Adduct of aquacobalamin with hydrogen peroxide. Inorganic Chemistry, 2021, 60(17),  12681-12684.<\/li>\n\n\n\n
  • Puscas, Cristina; Moldovan, Marioara; Silaghi-Dumitrescu, Laura; Ungureanu, Lavinia; Silaghi-Dumitrescu Radu. On the apparent redox reactivity of \u201coxygen-enriched water\u201d. Biological Trace Element Research, 2020, 198, 350\u2013358. doi.org\/10.1007\/s12011-020-02056-4.<\/li>\n\n\n\n
  • Mot, Augustin C.; Coman, Cristina; Hadade, Niculina; Damian, Grigore; Silaghi-Dumitrescu, Radu; Heering, Hendrik A. \u201cYellow\u201d laccase from Sclerotinia sclerotiorum is a blue laccase that enhances its substrate affinity by forming a reversible tyrosyl-product adduct. PLoS One, 2019, 0225530.<\/li>\n\n\n\n
  • Branzanic, Adrian; Ryde, Ulf; Silaghi-Dumitrescu, Radu. Why does sulfite reductase employ siroheme? Chemical Communications, 2019, 55, 14047-14049.<\/li>\n\n\n\n
  • Puscas, Cristina; Mircea, Alina; Raiu, Madalina; Mic, Mihaela; Attia, Amr A. A.; Silaghi-Dumitrescu Radu. Affinity and effect of anticancer drugs on the redox reactivity of hemoglobin. Chemical Research in Toxicology, 2019, 32(7), 1402-1411.<\/li>\n\n\n\n
  • Mot, Augustin C; Puscas, Cristina; Dorneanu, Sorin; Silaghi-Dumitrescu Radu. EPR detection of sulfanyl radical during sulfhemoglobin formation\u2013Influence of catalase. Free Radical Biology and Medicine, 2019, 137, 110-115.<\/li>\n\n\n\n
  • Turcas, Ramona; Kripli, Bal\u00e1zs; Attia, Amr A. A.; Lakk-Bog\u00e1th, D\u00f3ra; Speier, G\u00e1bor; Giorgi, Michel; Silaghi-Dumitrescu, Radu; Kaizer, J\u00f3zsef. Catalytic and stoichiometric flavanone oxidation mediated by nonheme oxoiron (IV) complexes as flavone synthase mimics: kinetic, mechanistic and computational studies. Dalton Transactions, 2018, 47, 14416-14420.<\/li>\n\n\n\n
  • Puscas, Cristina; Radu, Luana; Carrascoza, Francisco; Mot, Augustin C; Amariei, Diana; Lungu, Oana; Scurtu, Florina; Podea, Paula; Septelean, Raluca; Matei, Alina; Mic, Mihaela; Attia, Amr A.; Silaghi-Dumitrescu Radu. The high affinity of small-molecule antioxidants for hemoglobin. Free Radical Biology and Medicine, 2018, 124, 260-274.<\/li>\n\n\n\n
  • Mot, Augustin C; Puscas, Cristina; Miclea, Patricia; Naumova-Letia, Galaba; Dorneanu, Sorin; Podar, Dorina; Dissmeyer, Nico; Silaghi-Dumitrescu Radu. Redox control and autoxidation of class 1, 2 and 3 phytoglobins from Arabidopsis thaliana. Scientific Reports, 2018, 8(1), 13714.<\/li>\n\n\n\n
  • Mot, Augustin; C.; Bischin, Cristina; Damian, Grigore; Attia, Amr A.A.; Gal, Emese; Dina, Nicoleta E.; Leopold, Nicolae; Silaghi-Dumitrescu, Radu. Fe(III) \u2013 sulfide interaction in globins: characterization and quest for a putative Fe(IV)-sulfide species. Journal of Inorganic Biochemistry, 2018, 179, 32-39.<\/li>\n\n\n\n
  • Dereven’kov, Ilia; Ivlev, Pavel; Bischin, Cristina; Salnikov, Denis; Silaghi-Dumitrescu, Radu; Makarov, Sergei; Koifman, Oscar. Comparative studies of reaction of cobalamin (ii) and cobinamide (ii) with sulfur dioxide. Journal of Biological Inorganic Chemistry, 2017, 22(2), 969-975.<\/li>\n\n\n\n
  • Bischin, Cristina; Attia, Amr A. A.; Silaghi-Dumitrescu, Radu. Chlorite reactivity with myoglobin: analogy with peroxide and nitrite chemistry? Journal of Inorganic Biochemistry, 2017, 172, 122-128.<\/li>\n\n\n\n
  • Hathazi, Denisa; Mot, Augustin C; Vaida, Anetta; Scurtu, Florina; Lupan, Iulia; Fischer-Fodor,  Eva; Damian, Grigore; Kurtz, Jr., Donald M.; Silaghi-Dumitrescu, Radu. Oxidative protection of hemoglobin and hemerythrin by cross-linking with a non-heme iron peroxidase: potentially improved oxygen carriers for use in blood substitutes. Biomacromolecules, 2014, 15(5), 1920-1927.<\/li>\n\n\n\n
  • Mot, Augustin; Kis, Zoltan; Svistunenko, Dimitri A.; Damian, Grigore; Makarov, Sergei V.; Silaghi-Dumitrescu, Radu. \u2018Super-reduced\u2019 iron under physiologically-relevant conditions. Dalton Transactions, 2010, 39(6):1464-6.<\/li>\n<\/ul>\n\n\n\n

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    Research centers The Laboratory of EPR spectroscopy is overseen jointly by researchers from the The Center for Fundamental and Applied Heterochemistry \u2013 Metallomica, the Computer …<\/p>\n","protected":false},"author":1,"featured_media":103,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[5],"tags":[],"class_list":["post-51","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-nmr-epr-platform"],"_links":{"self":[{"href":"https:\/\/research.ubbcluj.ro\/infrastructure\/wp-json\/wp\/v2\/posts\/51","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/research.ubbcluj.ro\/infrastructure\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/research.ubbcluj.ro\/infrastructure\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/research.ubbcluj.ro\/infrastructure\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/research.ubbcluj.ro\/infrastructure\/wp-json\/wp\/v2\/comments?post=51"}],"version-history":[{"count":2,"href":"https:\/\/research.ubbcluj.ro\/infrastructure\/wp-json\/wp\/v2\/posts\/51\/revisions"}],"predecessor-version":[{"id":105,"href":"https:\/\/research.ubbcluj.ro\/infrastructure\/wp-json\/wp\/v2\/posts\/51\/revisions\/105"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/research.ubbcluj.ro\/infrastructure\/wp-json\/wp\/v2\/media\/103"}],"wp:attachment":[{"href":"https:\/\/research.ubbcluj.ro\/infrastructure\/wp-json\/wp\/v2\/media?parent=51"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/research.ubbcluj.ro\/infrastructure\/wp-json\/wp\/v2\/categories?post=51"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/research.ubbcluj.ro\/infrastructure\/wp-json\/wp\/v2\/tags?post=51"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}